112
so that, probably, the infection occurred further north. Austen in his Monograph, p. 326, records that Glossina fusca has been found on the north-east shores of Lake Rudolph. Vide Map of Distribution of Tsetse Flies in Africa (p. 282).
D. A mule used by Colonel Sadler was found in September, 1903, to have trypanosomes in his blood. This animal had been about five years in Africa, firstly, in the East Africa Protectorate, and for the last eighteen months in Uganda.
14. Can any difference be made out microscopically between these varieties of trypanosomes occurring amongst the domestic animals in Uganda ?
As the same species of trypanosoma varies in size, shape, etc., in the blood of different experimental animals, too much importance cannot be attached to the morphological characters as affording a means of establishing the identity of different trypanosomes. Speaking generally, it may be safely stated that the trypanosomes found in the blood and cerebrospinal fluid of cases of sleeping sickness, and in the blood of men showing no signs of sleeping sickness, are always smaller than those of the Jmja cattle, Abyssinian or Entebbe mule disease. The variety met with in the mule showed an unusually large number of short “ tadpole ” forms. This was especially well seen when the blood containing this variety was injected into a guinea pig (see p. 154). The variety met with in the Jinja cattle was, as a rule, larger than the others. It is, however, on morphological grounds only, impossible to arrive at a final conclusion as to the identity or otherwise of the various “ strains ” brought under our notice. Accordingly, in addition to this means of distinction, their differentiation was approached by a study of their reactions in a series of animals. These reactions were contrasted and compared. Further, the injection of animals proved to be immune to one species with the blood containing another strain of trypanosoma, was used as a means of arriving at a conclusion on the question of the identity or not of the various species. In the drawing of the specimens the morphological characters of the trypanosomes are shown.
15. Are these trypanosomes pathogenic to animals, and can any difference be made out between them by animal experiment ?
In the case of the trypanosoma found in Mr. Pordage’s ox, it produced a very chronic malady in the animals under observation, the animals became extremely emaciated with abnormal temperature. They became gradually weaker and finally died.
The trypanosoma obtained from the Jinja cattle produced few symptoms. There was a general enlargement of the lymphatic glands. As a rule the animals died in fairly good