fluid in pleural or peritoneal cavities, slight increase in pericardial.
Brain. —On removing the calvarium the dura mater is seen to be normal; on reflecting it, the convolutions are seen to be slightly flattened and the superficial vessels are injected; the sub-arachnoid fluid is increased—no active trypanosomes were seen microscopically, but the animal had been dead for some time. A pure culture of a streptococcus was obtained from the cerebro-spinal fluid.
Heart. — Nothing noteworthy. Blood from this organ examined microscopically shoAved many trypanosomes. Malaria is also present.
Bungs .—Are both healthy.
Liver, Spleen, and Kidneys.Show nothing noteAvorthy.
fntestines. —Are healthy.
Lymph Glands. — In omentum and mesentery distinctly enlarged.
Remarks. —This experiment demonstrates several points of importance. The first being that it is possible to commy the trypanosoma of sleeping sickness from man to monkey after an interval of 24 hours. »Secondly, that the disease produced in the monkey by the fly infection presents the same characters as that produced by inoculation of cerebro-spinal fluid or blood from a case of sleeping sickness. This animal presented toAA r ards the close of its life a typical picture of a sleeping sickness case.
This experiment is, finally, of interest ami importance from the fact that 15 days before its death it had been injected Avitli a pure culture of diplococci obtained from a case of sleeping sickness. So far as Ave could obseiwe, the course of the disease Avas uninfluenced by the injection, the only noteAvorthy feature being a slight suppuration in one of the groups of lymphatic glands near the site of inoculation. Portions of the neiwous system and glands haA^e been preseiwed for minute investigation and the results of the examination Avili be of interest.
8. Has the so-called Trypanosoma Fever any connection ivith Sleeping Sickness?
Since the publication of the last Report the obseiwations on the five men in Avhose blood the trypanosomes Avere first discovered in March, 1903, have been continued.
Tavo of these, Karala Barigi and Bara Risgallah, died of pneumonia in April and May, 1904, respectiA r ely ; of the others, .lordien Murjan appears to be undoubtedly in an early stage of sleeping sickness. He has gradually deA r eloped the characteristic signs of the malady. Trypanosomes are iioav ahvavs found in his cerebro-spinal fluid. Tabula presents some of the features of the disease, but is still able to do his work and has not yet shoAvn trypanosomes in the cerebro-spinal fluid.* Kumsarsabba is in a similar condition.
* Lieut. Gray writes, February, 1905, “That Tabula now shows trypano- Roines in the cerebro-spinal fluid, and distinct signs of sleeping sickness.”