9. Arc these trypanosomes pathogenic to animals, and can any specific difference he made out between them by animal experiment?
The experiments on the varions animals have been continued throughout the year. The additional observations and results obtained strengthen and support the conclusions arrived at in the last report.
The monkey is the most satisfactory animal for experimental inoculation. The continued observations show that the effect produced in them is in all respects similar, whether the trypanosoma infection is produced by blood from so-called “trypanosoma fever” cases or the cerebro-spiual fluid of undoubted sleeping sickness cases. As the question of the relationship of these two morbid conditions is an important one, full details of the experiments are given.
The other animals that we have employed for experimental inoculation are dogs, jackals, cats, rats, guinea-pigs, rabbits, oxen, goats, sheep and donkeys. None of these have shown any marked susceptibility to the disease, and some have remained resistant.
A. Experiments on the effect on monkeys of the injections of cerebro-spinal fluid containing trypanosomes taken by lumbar puncture from cases of sleeping sickness.
Experiment 2. Monkey (Male) (Macacus Rhesus).
March 23,’ 1903. Injected sediment of about 10 c.c. of cerebro-spinal fluid taken post-mortem from Case 18, Kapere.
May 11. Injected 2 c.c. cerebro-spinal fluid.
May 21. Trypanosomes are found in the blood to-day, 10 days after the second inoculation.
August 25. No marked symptoms up to the present.
November 28. Animal has been getting distinctly emaciated. He looks ill, but is able to rise.
The temperature curve remained about normal from March till August, 1903. From September, 1903, the evening temperature rose to about 103°, 105°, and fell in the morning to about 100°. The day before death, December 2, 1903, it fell to 94-8°.
The following table shows the presence or absence of trypanosomes in the blood and cerebro-spinal fluid :—